PT  - JOURNAL ARTICLE
AU  - Domínguez, Clara
AU  - López, Ana
AU  - Ramos-Cabrer, Pedro
AU  - Vieites-Prado, Alba
AU  - Pérez-Mato, Maria
AU  - Villalba, Carmen
AU  - Sobrino, Tomás
AU  - Rodriguez-Osorio, Xiana
AU  - Campos, Francisco
AU  - Castillo, José
AU  - Leira, Rogelio
TI  - Iron deposition in periaqueductal gray matter as a potential biomarker for chronic migraine
AID  - 10.1212/WNL.0000000000007047
DP  - 2019 Mar 05
TA  - Neurology
PG  - e1076--e1085
VI  - 92
IP  - 10
4099  - http://n.neurology.org/content/92/10/e1076.short
4100  - http://n.neurology.org/content/92/10/e1076.full
SO  - Neurology2019 Mar 05; 92
AB  - Objective To study iron deposition in red nucleus (RN), globus pallidus (GP), and periaqueductal gray matter (PAG) as a potential biomarker of chronic migraine (CM) and its association with levels of biomarkers related to migraine pathophysiology.Methods This case-control study included 112 patients with migraine (55 CM, 57 episodic migraine [EM]) and 25 headache-free controls. We analyzed iron deposition using 3T MRI and the NIH software platform ImageJ; we analyzed serum levels of markers of inflammation, endothelial dysfunction, and blood-brain barrier (BBB) disruption by ELISA in peripheral blood during interictal periods.Results Patients with CM showed larger iron grounds volume in RN compared to patients with EM (70.2 ± 6.8 vs 25.5 ± 7.3 μL, p < 0.001) and controls (70.2 ± 6.8 vs 15.1 ± 10.8 μL, p < 0.001), as well as larger iron deposits in PAG compared to patients with EM (360.3 ± 6.5 vs 249.7 ± 6.9 μL, p < 0.001) and controls (360.3 ± 6.5 vs 168.6 ± 10.3 μL, p < 0.001). In PAG, differences were also significant between patients with EM and controls. No significant differences were obtained for GP. Receiver operating characteristic curves showed that the optimal threshold for iron volume was 15 μL in RN (80% sensitivity, 71% specificity) and 240 μL in PAG (93% sensitivity, 97% specificity). Iron grounds volume in PAG was correlated with higher plasma levels of soluble tumor necrosis factor–like WEAK (r = 0.395, p = 0.005) and cellular fibronectin (r = 0.294, p = 0.040).Conclusions Patients with CM showed increased iron deposition in RN and PAG compared to patients with EM and controls. Iron grounds volume in PAG identified correctly patients with CM and was associated with elevated biomarkers of endothelial dysfunction and BBB disruption.BBB=blood-brain barrier; BG=basal ganglia; CAMERA=Cerebral Abnormalities in Migraine, an Epidemiological Risk Analysis; cFn=cellular fibronectin; CGRP=calcitonin gene-related peptide; EM=episodic migraine; GP=globus pallidus; hs-CRP=high-sensitivity C-reactive protein; IL=interleukin; PAG=periaqueductal gray matter; PTX3=pentraxin 3; RN=red nucleus; ROI=region of interest; sTWEAK=soluble tumor necrosis factor–like WEAK; TGV=trigeminovascular; TNF-α=tumor necrosis factor-α