PT  - JOURNAL ARTICLE
AU  - Fratini, Federica
AU  - Principe, Serena
AU  - Puopolo, Maria
AU  - Ladogana, Anna
AU  - Poleggi, Anna
AU  - Piscopo, Paola
AU  - Bruno, Giuseppe
AU  - Castrechini, Silvano
AU  - Pascone, Roberto
AU  - Confaloni, Annamaria
AU  - Minghetti, Luisa
AU  - Cardone, Franco
AU  - Pocchiari, Maurizio
AU  - Crescenzi, Marco
TI  - Increased levels of acute-phase inflammatory proteins in plasma of patients with sporadic CJD
AID  - 10.1212/WNL.0b013e318265a55d
DP  - 2012 Sep 04
TA  - Neurology
PG  - 1012--1018
VI  - 79
IP  - 10
4099  - http://n.neurology.org/content/79/10/1012.short
4100  - http://n.neurology.org/content/79/10/1012.full
SO  - Neurology2012 Sep 04; 79
AB  - Objective: Screening plasma samples from patients with sporadic Creutzfeldt-Jakob disease (CJD) to discover diagnostic biomarkers. Methods: Plasma samples were collected from 17 patients with sporadic CJD, 17 patients with Alzheimer disease (AD), and 20 healthy subjects. A 2-phase screening was carried out using quantitative protein mass spectrometry. The putative sporadic CJD biomarkers were then validated independently by immunoturbidimetry. Results: Mass spectrometry uncovered 7 candidate sporadic CJD protein biomarkers, all belonging to the acute-phase response. Highly significant increases of these markers in patients with sporadic CJD, compared with healthy subjects and patients with AD, was confirmed by immunoturbidimetry. Conclusions: The increase in plasma levels of a related set of acute-phase reactants in patients with sporadic CJD is a novel finding that suggests new pathogenetic hypotheses. The possible value of this set of proteins as biomarkers in the diagnosis of sporadic CJD or for blood/tissue donor screening remains to be further explored and validated in larger studies. AAT=α1-antitrypsin; AD=Alzheimer disease; AGP=α1-acid glycoprotein; AUC=area under the receiver operating characteristic curve; brAD=bedridden subjects with Alzheimer disease; CI=confidence interval; CJD=Creutzfeldt-Jakob disease; DSM-IV=Diagnostic and Statistical Manual of Mental Disorders, 4th edition; FG=fibrinogen; IL=interleukin; nonbrAD=non-bedridden subjects with Alzheimer disease; ROC=receiver operating characteristic; TSE=transmissible spongiform encephalopathy