RT Journal Article
SR Electronic
T1 Increased levels of acute-phase inflammatory proteins in plasma of patients with sporadic CJD
JF Neurology
JO Neurology
FD Lippincott Williams & Wilkins
SP 1012
OP 1018
DO 10.1212/WNL.0b013e318265a55d
VO 79
IS 10
A1 Fratini, Federica
A1 Principe, Serena
A1 Puopolo, Maria
A1 Ladogana, Anna
A1 Poleggi, Anna
A1 Piscopo, Paola
A1 Bruno, Giuseppe
A1 Castrechini, Silvano
A1 Pascone, Roberto
A1 Confaloni, Annamaria
A1 Minghetti, Luisa
A1 Cardone, Franco
A1 Pocchiari, Maurizio
A1 Crescenzi, Marco
YR 2012
UL http://n.neurology.org/content/79/10/1012.abstract
AB Objective: Screening plasma samples from patients with sporadic Creutzfeldt-Jakob disease (CJD) to discover diagnostic biomarkers. Methods: Plasma samples were collected from 17 patients with sporadic CJD, 17 patients with Alzheimer disease (AD), and 20 healthy subjects. A 2-phase screening was carried out using quantitative protein mass spectrometry. The putative sporadic CJD biomarkers were then validated independently by immunoturbidimetry. Results: Mass spectrometry uncovered 7 candidate sporadic CJD protein biomarkers, all belonging to the acute-phase response. Highly significant increases of these markers in patients with sporadic CJD, compared with healthy subjects and patients with AD, was confirmed by immunoturbidimetry. Conclusions: The increase in plasma levels of a related set of acute-phase reactants in patients with sporadic CJD is a novel finding that suggests new pathogenetic hypotheses. The possible value of this set of proteins as biomarkers in the diagnosis of sporadic CJD or for blood/tissue donor screening remains to be further explored and validated in larger studies. AAT=α1-antitrypsin; AD=Alzheimer disease; AGP=α1-acid glycoprotein; AUC=area under the receiver operating characteristic curve; brAD=bedridden subjects with Alzheimer disease; CI=confidence interval; CJD=Creutzfeldt-Jakob disease; DSM-IV=Diagnostic and Statistical Manual of Mental Disorders, 4th edition; FG=fibrinogen; IL=interleukin; nonbrAD=non-bedridden subjects with Alzheimer disease; ROC=receiver operating characteristic; TSE=transmissible spongiform encephalopathy