Histopathologic analysis of mice treated with estriol or placebo
| EAE mice treatment | No. demyelinated lesions | No. inflammatory lesions |
|---|---|---|
| Histopathologic analysis of mice given 90-day-release implants of estriol (5 or 15 mg) or placebo before the adoptive transfer of myelin basic protein-specific cells to induce EAE. Three mice were examined in each treatment group. Two blocks of lumbosacral spinal cord from each mouse, separated spatially by 1 to 2 mm, underwent semiquantitative assessment for demyelination and inflammation. Inflammation occurred in perivascular locations. In addition to a decrease in demyelination and inflammation, estriol-treated mice demonstrated markedly decreased meningeal infiltration. Mice were sacrificed at the day of maximal clinical disease difference between placebo and estriol treatment, with placebo mouse 1 at clinical grade 2.5, placebo mice 2 and 3 at clinical grade 4.0, and all three 5-mg estriol-treated mice and all three 15-mg estriol-treated mice at clinical grade 0. | ||
| EAE = experimental autoimmune encephalomyelitis. | ||
| Placebo treated | ||
| Placebo 1 | 9 | 5 |
| Placebo 2 | 13 | 21 |
| Placebo 3 | 10 | 12 |
| Estriol treated, 5 mg | ||
| Estriol 1 | 1 | 0 |
| Estriol 2 | 0 | 0 |
| Estriol 3 | 0 | 0 |
| Estriol treated, 15 mg | ||
| Estriol 1 | 0 | 0 |
| Estriol 2 | 0 | 0 |
| Estriol 3 | 0 | 0 |